The author offers an overview of pollen biology and biotechnology for students and researchers in areas such as reproductive biology, biotechnology, aeropalynology, plant breeding, horticulture, and forestry. Citing more than 1,500 references to pollen research, the text covers topics including advances in understanding pollen tube growth, the use of pollen for gene transfer, and advantages and disadvantages of various pollination systems for production of species limits.

The author offers an overview of pollen biology and biotechnology for students and researchers in areas such as reproductive biology, biotechnology, aeropalynology, plant breeding, horticulture, and forestry. Citing more than 1,500 references to pollen research, the text covers topics including advances in understanding pollen tube growth, the use of pollen for gene transfer, and advantages and disadvantages of various pollination systems for production of species limits.

Successful reproduction is the basis not only for the stability of the species in their natural habitat but also for productivity of our crop plants. Therefore, knowledge on reproductive ecology of wild and cultivated plants is important for effective management of our dwindling biodiversity and for the sustainability and improvement of the yield in crop species. Conservation and management of our plant diversity is going to be a major challenge in the coming decades, particularly in the tropical countries which are rich in biodiversity. Reproductive failure is the main driver for pushing a large number of tropical species to vulnerable category. Available data on reproductive ecology on tropical species is very limited and there is an urgent need to initiate research on these lines. A major limitation for the beginners to take up research is the absence of simple concise work manuals that provide step-wise procedures to study all aspects of reproductive ecology. The Manual fills this void. Over 60 protocols described in the manual cover the whole spectrum of reproductive ecology - study sites and species, phenology, floral morphology and sexuality, pollen and pistil biology, pollination ecology, breeding system, seed biology, seed dispersal and seedling recruitment. Each chapter gives a concise conceptual account of the topic before describing the protocols. The Manual caters to researchers, teachers and students who are interested in any aspect of reproductive ecology of flowering plants -- botanists, ecologists, agri-horticulturists, foresters, entomologists, plant breeders and conservation biologists.

Successful reproduction is the basis not only for the stability of the species in their natural habitat but also for productivity of our crop plants. Therefore, knowledge on reproductive ecology of wild and cultivated plants is important for effective management of our dwindling biodiversity and for the sustainability and improvement of the yield in crop species. Conservation and management of our plant diversity is going to be a major challenge in the coming decades, particularly in the tropical countries which are rich in biodiversity. Reproductive failure is the main driver for pushing a large number of tropical species to vulnerable category. Available data on reproductive ecology on tropical species is very limited and there is an urgent need to initiate research on these lines. A major limitation for the beginners to take up research is the absence of simple concise work manuals that provide step-wise procedures to study all aspects of reproductive ecology. The Manual fills this void. Over 60 protocols described in the manual cover the whole spectrum of reproductive ecology - study sites and species, phenology, floral morphology and sexuality, pollen and pistil biology, pollination ecology, breeding system, seed biology, seed dispersal and seedling recruitment. Each chapter gives a concise conceptual account of the topic before describing the protocols. The Manual caters to researchers, teachers and students who are interested in any aspect of reproductive ecology of flowering plants -- botanists, ecologists, agri-horticulturists, foresters, entomologists, plant breeders and conservation biologists.

Reproductive biology is the basis of species improvement and a thorough understanding of this is needed for plant improvement, whether by conventional or biotechnological methods. This book presents an up to date and comprehensive description of reproduction in lower plants, gymnosperms and higher plants. It covers general plant biology, pollination, pollen-pistil interaction, post-fertilization changes, and seed dormancy.

Pollen grains are everywhere - in the air, in the water, in soil and in the food we eat. Pollen has direct relevance in agriculture, horticulture, plant breeding, crop improvement and biotechnology. They are further of use for montioring cytotoxic effects - by herbicides, pesticides and pollutants - testing for allergic reactions, and for basic studies on gene expression, research on differentiation and polarity. Detailed instructions of the standard techniques, which have all been tested and improvised by the authors, are given, such as collection and storage of pollen, pollen culture, germination, tests for viability, incompatibility and isolation of protoplasts. Introduced by an explanation of the principles involved, the step-by step protocols are complemented by personal notes and precautions, specifying the reagents used and various appendices on basic and specific requirements for laboratory exercises on pollen.

Our knowledge of pollen, the gold dust that carries the male germ line of flowering plants and is vital for sexual reproduction and seed formation, has 'come of age' with the publication of this book. Here, for the first time in a single volume, are all the ideas and techniques developed in the last two decades concerning the manipulation of pollen and pollen tubes in plant breeding and biotechnology. Pollen has never been an easy topic to come to grips with, with its variable and often inexplicable terminology that has made it a more difficult field in which to work. This book will remedy that, with its overview of pollen biology and pollen-pistil interactions that explains terms and concepts of the male function of pollen in a way that is readily understandable to the student and professional plant scientist and plant breeder.